Relion 5.0 Aretomo not working

[dadmer]

Describe your problem

Hello, I have an issue trying to align my tomograms using Aretomo in Relion 5.
Alignment using Imod works but does not give me satisfactory results, I also have Aretomo previously installed and it works fine.

Environment:

• OS: [Ubuntu 22.04.4 LTS]
• MPI runtime: [4.1.1]
• RELION version [RELION-5.0-beta-2-commit-a0b145
• Memory: [128 GB]
• GPU: [RTX 3090]

Job options:

• Full command :

  ``which relion_python_tomo_align_tilt_series` AreTomo  --sample-thickness-nanometers 200 --do-tilt-angle-offset-correction  --tilt-series-star-file ExcludeTiltImages/job004/selected_tilt_series.star --output-directory AlignTiltSeries/job034/  --pipeline_control AlignTiltSeries/job034/
  

Error message:

[12:22:24] ERROR: alignment for Position_11 failed       align_tilt_series.py:70
[12:22:30] ERROR: alignment for Position_15 failed       align_tilt_series.py:70
[12:22:36] ERROR: alignment for Position_16 failed       align_tilt_series.py:70
[12:22:42] ERROR: alignment for Position_21 failed       align_tilt_series.py:70
[12:22:48] ERROR: alignment for Position_22 failed       align_tilt_series.py:70
[12:22:54] ERROR: alignment for Position_5 failed        align_tilt_series.py:70
[12:23:01] ERROR: alignment for Position_6 failed        align_tilt_series.py:70

==================

Thank you very much for your help,
David

[Amy1809]

Hi Daivd, I have the same problem as you where it gives me error of failed alignment at algin_tilti_series.py:70. Do you have any solutions on this now?