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AreTomo executable was not found #1150
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Hi @lmcarreira, You need to make sure that you have AreTomo installed and that the binary is available in your
If you are on an HPC cluster where AreTomo is available as a module, make sure to load that module before launching RELION. |
Hi @rdrighetto, thanks for the reply.
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Are you submitting the job to a cluster e.g. with SLURM? If so, you have to make sure that the AreTomo module (or Furthermore, is the binary located in |
This is a template for reporting bugs. Please fill in as much information as you can.
Describe your problem
I was trying to run the new Relion 5 Tomo but unfortunately it seems not to find AreTomo to perform tilt-series alignements.
Job options:
note.txt
in the job directory):which relion_python_tomo_align_tilt_series
AreTomo --sample-thickness-nanometers 100 --gpu 1 --tilt-series-star-file ExcludeTiltImages/job021/selected_tilt_series.star --output-directory AlignTiltSeries/job028/ --pipeline_control AlignTiltSeries/job028/Error message:
Please cite the full error message as the example below.
/home/lucarreir/miniconda3/envs/relion-5.0/lib/python3.10/site-packages/starfile/parser.py:177: FutureWarning: The 'delim_whitespace' keyword in pd.read_csv is deprecated and will be removed in a future version. Use
sep='\s+'
insteaddf = pd.read_csv(
/home/lucarreir/miniconda3/envs/relion-5.0/lib/python3.10/site-packages/starfile/parser.py:194: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing
errors
and catch exceptions explicitly insteadself.dataframes[key] = df.apply(pd.to_numeric, errors='ignore')
/home/lucarreir/miniconda3/envs/relion-5.0/lib/python3.10/site-packages/starfile/parser.py:177: FutureWarning: The 'delim_whitespace' keyword in pd.read_csv is deprecated and will be removed in a future version. Use
sep='\s+'
insteaddf = pd.read_csv(
/home/lucarreir/miniconda3/envs/relion-5.0/lib/python3.10/site-packages/starfile/parser.py:194: FutureWarning: errors='ignore' is deprecated and will raise in a future version. Use to_numeric without passing
errors
and catch exceptions explicitly insteadself.dataframes[key] = df.apply(pd.to_numeric, errors='ignore')
[14:25:31] ERROR: alignment for TS_030 failed with align_tilt_series.py:73
error: AreTomo executable was not found.
Put 'AreTomo' on the PATH to proceed.
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