Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

correct insertion extraction from mpileup #175

Open
tdelhomme opened this issue May 9, 2018 · 1 comment
Open

correct insertion extraction from mpileup #175

tdelhomme opened this issue May 9, 2018 · 1 comment
Assignees
Labels
bug external need resolving the issue needs external updates on dependencies
Milestone

Comments

@tdelhomme
Copy link
Member

At the moment, samtools mpileup gives an "=" at the end of some insertions, and we have opened an issue for that.

This results in such a line in the VCF, were ALT contains (or is) an equal:

#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	IonXpress_005	IonXpress_019	IonXpress_020	IonXpress_030	IonXpress_053	IonXpress_104	IonXpress_105
chr11	62397115	.	-	=	152.793	.	TYPE=ins;NS=103;AF=0.00970874;DP=43980;RO=37749;AO=10;SRF=17260;SRR=20489;SAF=5;SAR=5;SOR=0.536285;RVSB=0.542758;FS=0;ERR=5.01679e-05;SIG=0.001;CONT=ATGxCCC;AC=0;AN=14	GT:QVAL:DP:RO:AO:AF:SB:SOR:RVSB:FS:QVAL_minAF:STATUS	0/0:0:202:177:0:0:112,65,0,0:-1:-1:0:1217.54:.	0/0:0:298:248:0:0:120,128,0,0:-1:-1:0:1850.99:.	0/0:0:306:244:0:0:99,145,0,0:-1:-1:0:1879.87:.	0/0:0:469:414:0:0:181,233,0,0:-1:-1:0:2952.58:.	0/0:0:401:349:0:0:138,211,0,0:-1:-1:0:2512.24:.	0/0:0:672:557:0:0:229,328,0,0:-1:-1:0:inf:.	0/0:0:713:623:0:0:322,301,0,0:-1:-1:0:inf:.

Either it is a bug from samtools and we just need to wait for their bug resolving or we need adapt the mpileup2readcounts script.

@tdelhomme tdelhomme added bug external need resolving the issue needs external updates on dependencies labels May 9, 2018
@tdelhomme tdelhomme added this to the v1.1 milestone May 9, 2018
@tdelhomme
Copy link
Member Author

tdelhomme commented May 9, 2018

This is actually an htslib 8yo bug reported here, for the moment we should adapt our mpileup2readcounts until they correct this.

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
bug external need resolving the issue needs external updates on dependencies
Projects
None yet
Development

No branches or pull requests

2 participants