diff --git a/mutmut/__init__.py b/mutmut/__init__.py
index 66f1cf0f..920b0cc5 100644
--- a/mutmut/__init__.py
+++ b/mutmut/__init__.py
@@ -1220,7 +1220,7 @@ def read_patch_data(patch_file_path: str):
         diffs = whatthepatch.parse_patch(f.read())
 
     return {
-        diff.header.new_path: {change.new for change in diff.changes if change.old is None}
+        os.path.normpath(diff.header.new_path): {change.new for change in diff.changes if change.old is None}
         for diff in diffs if diff.changes
     }
 
diff --git a/tests/test_init.py b/tests/test_init.py
index b5c236ed..6111fd30 100644
--- a/tests/test_init.py
+++ b/tests/test_init.py
@@ -1,4 +1,5 @@
 
+import os
 from pathlib import Path
 from time import sleep
 from pytest import raises, fixture
@@ -140,6 +141,18 @@ def test_read_patch_data_edited_line_is_in_the_list(testpatches_path: Path):
     assert file_name in file_changes
     assert file_changes[file_name] == {2} # line is added between 2nd and 3rd
 
+def test_read_patch_data_edited_line_in_subfolder_is_in_the_list(testpatches_path: Path):
+    # arrange
+    file_name = os.path.join("sub", "existing_file.txt") # unix will use "/", windows "\" to join
+    file_patch = testpatches_path / "edit_existing_line_in_subfolder.patch"
+
+    # act
+    file_changes = read_patch_data(file_patch)
+
+    # assert
+    assert file_name in file_changes
+    assert file_changes[file_name] == {2} # line is added between 2nd and 3rd
+
 def test_read_patch_data_renamed_file_edited_line_is_in_the_list(testpatches_path: Path):
     # arrange
     original_file_name = "existing_file.txt"
diff --git a/tests/testdata/test_patches/edit_existing_line_in_subfolder.patch b/tests/testdata/test_patches/edit_existing_line_in_subfolder.patch
new file mode 100644
index 00000000..a6bae940
--- /dev/null
+++ b/tests/testdata/test_patches/edit_existing_line_in_subfolder.patch
@@ -0,0 +1,10 @@
+diff --git a/sub/existing_file.txt b/sub/existing_file.txt
+index 272b2e4..9767587 100644
+--- a/sub/existing_file.txt
++++ b/sub/existing_file.txt
+@@ -1,3 +1,3 @@
+ first line
+-second line
++second line is edited
+ third line
+\ No newline at end of file