diff --git a/MANIFEST.in b/MANIFEST.in
index 834ddfad39c6..919cd8a1cdb8 100644
--- a/MANIFEST.in
+++ b/MANIFEST.in
@@ -7,7 +7,6 @@ include demo/README
 include demo/demo.tls.dh
 include demo/*.py
 include demo/*.sh
-include sytest-blacklist
 
 recursive-include synapse/storage/schema *.sql
 recursive-include synapse/storage/schema *.sql.postgres
@@ -34,6 +33,7 @@ exclude Dockerfile
 exclude .dockerignore
 exclude test_postgresql.sh
 exclude .editorconfig
+exclude sytest-blacklist
 
 include pyproject.toml
 recursive-include changelog.d *
diff --git a/changelog.d/5733.misc b/changelog.d/5733.misc
new file mode 100644
index 000000000000..a2a8c26383f6
--- /dev/null
+++ b/changelog.d/5733.misc
@@ -0,0 +1 @@
+Don't package the sytest test blacklist file.