diff --git a/instrumentation/opentelemetry-instrumentation-mysqlclient/pyproject.toml b/instrumentation/opentelemetry-instrumentation-mysqlclient/pyproject.toml
index de8c31a1f3..7646a9be2b 100644
--- a/instrumentation/opentelemetry-instrumentation-mysqlclient/pyproject.toml
+++ b/instrumentation/opentelemetry-instrumentation-mysqlclient/pyproject.toml
@@ -33,10 +33,6 @@ dependencies = [
 instruments = [
   "mysqlclient < 3",
 ]
-test = [
-  "opentelemetry-instrumentation-mysqlclient[instruments]",
-  "opentelemetry-test-utils == 0.45b0.dev",
-]
 
 [project.entry-points.opentelemetry_instrumentor]
 mysqlclient = "opentelemetry.instrumentation.mysqlclient:MySQLClientInstrumentor"
diff --git a/instrumentation/opentelemetry-instrumentation-mysqlclient/test-requirements.txt b/instrumentation/opentelemetry-instrumentation-mysqlclient/test-requirements.txt
new file mode 100644
index 0000000000..afa2ccae6c
--- /dev/null
+++ b/instrumentation/opentelemetry-instrumentation-mysqlclient/test-requirements.txt
@@ -0,0 +1,19 @@
+asgiref==3.7.2
+attrs==23.2.0
+Deprecated==1.2.14
+importlib-metadata==6.11.0
+iniconfig==2.0.0
+mysqlclient==2.2.4
+packaging==23.2
+pluggy==1.4.0
+py==1.11.0
+py-cpuinfo==9.0.0
+pytest==7.1.3
+pytest-benchmark==4.0.0
+tomli==2.0.1
+typing_extensions==4.9.0
+wrapt==1.16.0
+zipp==3.17.0
+-e opentelemetry-instrumentation
+-e instrumentation/opentelemetry-instrumentation-dbapi
+-e instrumentation/opentelemetry-instrumentation-mysqlclient
diff --git a/tox.ini b/tox.ini
index dbd1a8ad15..8ac6a4606f 100644
--- a/tox.ini
+++ b/tox.ini
@@ -382,7 +382,7 @@ commands_pre =
 
   mysql: pip install {toxinidir}/instrumentation/opentelemetry-instrumentation-dbapi {toxinidir}/instrumentation/opentelemetry-instrumentation-mysql[test]
 
-  mysqlclient: pip install {toxinidir}/instrumentation/opentelemetry-instrumentation-dbapi {toxinidir}/instrumentation/opentelemetry-instrumentation-mysqlclient[test]
+  mysqlclient: pip install -r {toxinidir}/instrumentation/opentelemetry-instrumentation-mysqlclient/test-requirements.txt
 
   pymemcache-0: pip install -r {toxinidir}/instrumentation/opentelemetry-instrumentation-pymemcache/test-requirements-0.txt
   pymemcache-1: pip install -r {toxinidir}/instrumentation/opentelemetry-instrumentation-pymemcache/test-requirements-1.txt
@@ -599,7 +599,7 @@ commands_pre =
   python -m pip install -e {toxinidir}/instrumentation/opentelemetry-instrumentation-pymysql[test]
   # prerequisite: follow the instructions here https://github.com/PyMySQL/mysqlclient#install
   # for your OS to install the required dependencies
-  python -m pip install -e {toxinidir}/instrumentation/opentelemetry-instrumentation-mysqlclient[test]
+  pip install -r {toxinidir}/instrumentation/opentelemetry-instrumentation-mysqlclient/test-requirements.txt
   python -m pip install -e {toxinidir}/instrumentation/opentelemetry-instrumentation-pymongo[test]
   pip install -r {toxinidir}/instrumentation/opentelemetry-instrumentation-elasticsearch/test-requirements-2.txt
   python -m pip install -e {toxinidir}/instrumentation/opentelemetry-instrumentation-asyncpg[test]