diff --git a/CHANGELOG.md b/CHANGELOG.md
index d64e641089..53e15bc564 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -15,6 +15,7 @@ We use *breaking* word for marking changes that are not backward compatible (rel
 
 - [#2416](https://github.com/thanos-io/thanos/pull/2416) Bucket: fixes issue #2416 bug in `inspect --sort-by` doesn't work correctly in all cases
 - [#2411](https://github.com/thanos-io/thanos/pull/2411) Query: fix a bug where queries might not time out sometimes due to issues with one or more StoreAPIs
+- [##2484](https://github.com/thanos-io/thanos/pull/2484) Query/Ruler: fix issue #2483, when web.route-prefix is set, it is added twice in http router prefix.
 
 ### Added
 
diff --git a/cmd/thanos/query.go b/cmd/thanos/query.go
index a21565c59a..2735cd76c2 100644
--- a/cmd/thanos/query.go
+++ b/cmd/thanos/query.go
@@ -8,7 +8,6 @@ import (
 	"fmt"
 	"math"
 	"net/http"
-	"path"
 	"strings"
 	"time"
 
@@ -357,7 +356,7 @@ func runQuery(
 
 		api := v1.NewAPI(logger, reg, engine, queryableCreator, enableAutodownsampling, enablePartialResponse, replicaLabels, instantDefaultMaxSourceResolution)
 
-		api.Register(router.WithPrefix(path.Join(webRoutePrefix, "/api/v1")), tracer, logger, ins)
+		api.Register(router.WithPrefix("/api/v1"), tracer, logger, ins)
 
 		srv := httpserver.New(logger, reg, comp, httpProbe,
 			httpserver.WithListen(httpBindAddr),
diff --git a/cmd/thanos/rule.go b/cmd/thanos/rule.go
index 3c021dec2b..33889f7c99 100644
--- a/cmd/thanos/rule.go
+++ b/cmd/thanos/rule.go
@@ -8,7 +8,6 @@ import (
 	"math/rand"
 	"net/http"
 	"net/url"
-	"path"
 	"path/filepath"
 	"strconv"
 	"strings"
@@ -576,7 +575,7 @@ func runRule(
 		ui.NewRuleUI(logger, reg, ruleMgr, alertQueryURL.String(), webExternalPrefix, webPrefixHeaderName).Register(router, ins)
 
 		api := v1.NewAPI(logger, reg, ruleMgr)
-		api.Register(router.WithPrefix(path.Join(webRoutePrefix, "/api/v1")), tracer, logger, ins)
+		api.Register(router.WithPrefix("/api/v1"), tracer, logger, ins)
 
 		srv := httpserver.New(logger, reg, comp, httpProbe,
 			httpserver.WithListen(httpBindAddr),