Clr data from time-resolved serial-femtosecond-crystallography

This page provides supporting data for the study on the ClR, a chloride ion pumping rhodospin. The ClR has a chromophore, retinal linked to a lysine on transmembrane helix-G (the 7th helix from the N-terminal of ClR). Retinal isoermiazation triggered by light absorption will drive the Chloride ion through the channel of ClR.

This study applied time-resolved crystallography method using the XFEL source at LCLS (SLAC, Menlo Park, CA). The structures of dark state (t=0) and five pumped states (t=1,2,50,100 ps) were determined to atomic resolutions (1.65A for dark, 1.85A for pumped states).

In this page, there are five groups of files, following the data processing steps:

1. Fourier difference maps (DMAP_obs) calculated from experimental data: |F_pump-F_dark|_obs * exp(i phi_dark);
2. Extrapolated maps (ExtraMap_obs) calculated from experimental data: (F_dark+N_ext * |F_pump-F_dark|_obs) * exp(i phi_dark)
3. Refined atomic models : dark, 1-100 ps
4. MTZ with structure factors : dark, 1-100 ps
5. Fourier difference maps (DMAP_calc) calculated from refined atomic models