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Fixed content going off slide
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royfrancis committed Mar 21, 2024
1 parent e99724e commit e370edd
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28 changes: 19 additions & 9 deletions topics/rnaseq/slide_rnaseq.qmd
Original file line number Diff line number Diff line change
Expand Up @@ -51,7 +51,7 @@ upid <- yaml::read_yaml(here("_quarto.yml"))$uppmax_project
## Workflow

::: {.columns}
::: {.column width="65%"}
::: {.column width="60%"}

![](assets/rnaseq_workflow.svg)

Expand Down Expand Up @@ -94,7 +94,7 @@ upid <- yaml::read_yaml(here("_quarto.yml"))$uppmax_project
:::

::: {.column}
![](assets/batch-effect.svg){width="80%"}
![](assets/batch-effect.svg){width="75%"}
:::
::::

Expand All @@ -109,9 +109,12 @@ upid <- yaml::read_yaml(here("_quarto.yml"))$uppmax_project

:::: {.columns}
::: {.column}
![](assets/rnaseq_library_prep.svg){width="90%"}

![](assets/rnaseq_library_prep.svg){width="85%"}

:::
::: {.column}

![](assets/rnaseq_read_type.svg)

- polyA selection / Ribosomal RNA depletion
Expand Down Expand Up @@ -163,6 +166,9 @@ upid <- yaml::read_yaml(here("_quarto.yml"))$uppmax_project

## Read QC

::: {.columns}
::: {.column width="65%"}

- Number of reads
- Per base sequence quality
- Per sequence quality score
Expand All @@ -175,13 +181,17 @@ upid <- yaml::read_yaml(here("_quarto.yml"))$uppmax_project
- Adapter content
- Kmer content

<img src="assets/qc.jpg" style="height:400px; position:fixed; right:0px; bottom: 0px; margin-right: 100px; margin-bottom: 300px;">
[FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/), [MultiQC](http://multiqc.info/), <https://sequencing.qcfail.com/>

[FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/), [MultiQC](http://multiqc.info/)
![](assets/qcfail.jpg){width="60%"}

<https://sequencing.qcfail.com/>
:::
::: {.column width="35%"}

![](assets/qc.jpg)

![](assets/qcfail.jpg){width="90%"}
:::
:::

## FastQC

Expand Down Expand Up @@ -275,6 +285,8 @@ Poor quality

![](assets/compare-aligners-speed.png){width="90%"}

@baruzzo2017simulation

:::
::: {.column width="60%"}

Expand All @@ -285,8 +297,6 @@ Poor quality
:::
::::

@baruzzo2017simulation

::: {.notes}

## Aligners time and RAM
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