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Fix some errors/typos and add more explanations.
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fcyu committed Jul 4, 2018
1 parent 6be0c23 commit 17bb260
Showing 1 changed file with 11 additions and 11 deletions.
22 changes: 11 additions & 11 deletions src/main/resources/parameter.def
Original file line number Diff line number Diff line change
@@ -1,4 +1,4 @@
# 2.1.7
# 2.1.8-af07e84
# The first line is the parameter file version. Do not change it.
thread_num = 0 # The thread number. Set to 0 to use all the CPU resources of the computer.
debug = 0 # For debug.
Expand All @@ -9,27 +9,27 @@ db = small+random50.fasta # The protein database.
database_type = UniProt # Different types have different fasta header patterns. Available values: UniProt, SwissProt, TAIR, ITAG, RefSeq, Others
missed_cleavage = 2 # Maximum number of allowed missed cleavage.
min_chain_length = 5 # Minimum length of a peptide chain.
max_chain_length = 50
max_chain_length = 50 # Maximum length of a peptide chain.

# Spectrum
C13_correction = 1 # 1 = perform correction; 0 = not.

# Tolerance
ms1_tolerance_unit = 1 # 0: Da; 1: ppm
ms1_tolerance = 10
mz_bin_size = 0.02 # Bin size in digitizing a MS/MS spectrum.
mz_bin_offset = 0 # Offset in digitizing a MS/MS spectrum.
mz_bin_size = 0.02 # Bin size in digitizing a MS/MS spectrum. e.g. high-resolution MS/MS: 0.02; low-resolution MS/MS: 1.0005
mz_bin_offset = 0 # Offset in digitizing a MS/MS spectrum. e.g. high-resolution MS/MS: 0; low-resolution MS/MS: 0.4

# Cross-linking parameter.
cl_mass = 138.0680796 # Cross-linker mass.
cl_mass = 138.0680796 # Cross-linker mass after reaction.
cl_type = 1 # Cross-linker type. 1 = Kn-Kn; 2 = C-C

# Varibale modification.
# Variable modification.
# Format: <mass> <residues> <binary>
# <binary> == 0/1
# If <binary> = 1, all the specified amino acids in a peptide are either modified or not. Normally used for chemical labelling.
# Binary modification is mutual exclusion with each other
var_mod1 = 15.99 M 0
var_mod1 = 15.9949 M 0
var_mod2 = 0.0 X 0
var_mod3 = 0.0 X 0
var_mod4 = 0.0 X 0
Expand All @@ -38,7 +38,7 @@ var_mod6 = 0.0 X 0
var_mod7 = 0.0 X 0
var_mod8 = 0.0 X 0
var_mod9 = 0.0 X 0
var_mod_max_num = 5 # max number of modified amino acids in a peptide. The max value is 5
var_mod_max_num = 5 # Maximum number of modified amino acids in a peptide. The maximum value is 5

# Fix modification
G = 0
Expand Down Expand Up @@ -66,22 +66,22 @@ O = 0
n = 0
c = 0

# Enzyme digestion specificities. Only support one enzyme at a time.
# Enzyme digestion specifications. Only support one enzyme at a time.
# enzyme name is cut from C-term cleavage site protection site
Trypsin 1 KR P
# Trypsin/P 1 KR -
# TrypsinR 1 R P
# LysC 1 K P
# ArgC 1 R P
# Chymotrypsin 1 FYW P
# Chymotrypsin 1 FYWL P
# GluC 1 DE P
# LysN 0 K -
# AspN 0 D -

# Advanced parameters.
# Don't change them unless necessary.
single_chain_t = 0 # Single chain score threshold.
cal_evalue = 0 # 1 = calculate e-value; 0 = not.
cal_evalue = 0 # 1 = calculate e-value; 0 = not. For small database (< 500 proteins), set it to 0; For large database, set it to 1.
ms1_bin_size = 0.001
delta_c_t = 0.00 # DeltaC threshold.
flanking_peaks = 0 # 1 = use flanking peaks; 0 = not.
Expand Down

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