notebooks/example.ipynb

{
 "cells": [
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "# Example code to create a SpliceMap"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 2,
   "metadata": {},
   "outputs": [],
   "source": [
    "import numpy as np\n",
    "import pandas as pd\n",
    "from kipoiseq.extractors import MultiSampleVCF\n",
    "from splicemap.dataclasses import Junction\n",
    "from splicemap import SpliceCountTable\n",
    "import matplotlib.pyplot as plt"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 3,
   "metadata": {},
   "outputs": [],
   "source": [
    "fasta_file = 'path_to_fasta_file.fa'\n",
    "gtf_file = 'path_to_gtf_file.gtf.gz'"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "## 1) Read in example file"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 4,
   "metadata": {},
   "outputs": [],
   "source": [
    "path = 'path_to_count_table_dir/Brain_Cortex_raw_count_table.csv'\n",
    "ct = SpliceCountTable.read_csv(path)"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "### 1.1) Infer Strand (if no strand)\n",
    "If strand information does not contained in count_table it can be infered by reference genome."
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 6,
   "metadata": {},
   "outputs": [],
   "source": [
    "ct.df['Strand'] = '.' # removes strand information"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 7,
   "metadata": {},
   "outputs": [],
   "source": [
    "ct.df['Strand'] = ct.df['Strand'].astype(\"category\")"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 8,
   "metadata": {},
   "outputs": [
    {
     "name": "stderr",
     "output_type": "stream",
     "text": [
      "100%|██████████| 1065286/1065286 [00:24<00:00, 44073.78it/s]\n",
      "/data/nasif12/home_if12/wagnern/Projects/gitlab_gagneurlab/splicemap/splicemap/count_table.py:222: SettingWithCopyWarning: \n",
      "A value is trying to be set on a copy of a slice from a DataFrame.\n",
      "Try using .loc[row_indexer,col_indexer] = value instead\n",
      "\n",
      "See the caveats in the documentation: https://pandas.pydata.org/pandas-docs/stable/user_guide/indexing.html#returning-a-view-versus-a-copy\n",
      "  for i in junctions\n"
     ]
    }
   ],
   "source": [
    "ct.infer_strand(fasta_file, progress=True)"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 9,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/html": [
       "<div>\n",
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       "        text-align: right;\n",
       "    }\n",
       "</style>\n",
       "<table border=\"1\" class=\"dataframe\">\n",
       "  <thead>\n",
       "    <tr style=\"text-align: right;\">\n",
       "      <th></th>\n",
       "      <th>Chromosome</th>\n",
       "      <th>Start</th>\n",
       "      <th>End</th>\n",
       "      <th>Strand</th>\n",
       "      <th>individual 1</th>\n",
       "      <th>individual 2</th>\n",
       "      <th>individual 3</th>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>junctions</th>\n",
       "      <th></th>\n",
       "      <th></th>\n",
       "      <th></th>\n",
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       "      <th></th>\n",
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       "  </thead>\n",
       "  <tbody>\n",
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       "      <td>Y</td>\n",
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       "      <td>-</td>\n",
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       "      <td>0</td>\n",
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       "    <tr>\n",
       "      <th>Y:58895447-58909682:-</th>\n",
       "      <td>Y</td>\n",
       "      <td>58895447</td>\n",
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       "      <td>-</td>\n",
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       "</table>\n",
       "<p>1065286 rows × 7 columns</p>\n",
       "</div>"
      ],
      "text/plain": [
       "                      Chromosome     Start       End Strand  individual 1  \\\n",
       "junctions                                                                   \n",
       "1:10895-10969:+                1     10895     10969      +             1   \n",
       "1:11211-12009:+                1     11211     12009      +             0   \n",
       "1:11671-12009:+                1     11671     12009      +             1   \n",
       "1:11844-12009:+                1     11844     12009      +             0   \n",
       "1:12227-12594:+                1     12227     12594      +             0   \n",
       "...                          ...       ...       ...    ...           ...   \n",
       "Y:58888289-58902586:+          Y  58888289  58902586      +             0   \n",
       "Y:58888289-58909682:-          Y  58888289  58909682      -             0   \n",
       "Y:58895447-58909682:-          Y  58895447  58909682      -             0   \n",
       "Y:58899006-58909682:-          Y  58899006  58909682      -             0   \n",
       "Y:58906143-58909682:-          Y  58906143  58909682      -             0   \n",
       "\n",
       "                       individual 2  individual 3  \n",
       "junctions                                          \n",
       "1:10895-10969:+                   0             0  \n",
       "1:11211-12009:+                   0             0  \n",
       "1:11671-12009:+                   0             1  \n",
       "1:11844-12009:+                   0             0  \n",
       "1:12227-12594:+                   0             0  \n",
       "...                             ...           ...  \n",
       "Y:58888289-58902586:+             0             0  \n",
       "Y:58888289-58909682:-             0             0  \n",
       "Y:58895447-58909682:-             0             0  \n",
       "Y:58899006-58909682:-             0             0  \n",
       "Y:58906143-58909682:-             0             0  \n",
       "\n",
       "[1065286 rows x 7 columns]"
      ]
     },
     "execution_count": 9,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "ct.df.iloc[:,:7].rename(columns={\n",
    "    ct.df.columns[4]: 'individual 1',\n",
    "    ct.df.columns[5]: 'individual 2',\n",
    "    ct.df.columns[6]: 'individual 3'\n",
    "})"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "## 2) Use quality filters to generate a SpliceMap"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "Notes for provided quality filters in the splicemap package:"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "### 2.1) Median filter\n",
    "We use 'event5_median_filter' and 'event3_median_filter', which filters for events/splice-sites that have a median count over a certain cutoff value. Note that results depend a lot on the choice of this cutoff value"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 10,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/plain": [
       "<matplotlib.legend.Legend at 0x147d1fee0610>"
      ]
     },
     "execution_count": 10,
     "metadata": {},
     "output_type": "execute_result"
    },
    {
     "data": {
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\n",
      "text/plain": [
       "<Figure size 432x288 with 1 Axes>"
      ]
     },
     "metadata": {
      "needs_background": "light"
     },
     "output_type": "display_data"
    }
   ],
   "source": [
    "cutoff = 5\n",
    "\n",
    "ct.plot_median_read_hist_event5()\n",
    "plt.axvline(np.log(cutoff), 0, 1, label='cutoff', c='red')\n",
    "plt.legend()"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 11,
   "metadata": {},
   "outputs": [],
   "source": [
    "ct_psi5 = ct.event5_median_filter(cutoff=cutoff)\n",
    "ct_psi3 = ct.event3_median_filter(cutoff=cutoff)"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "`event5_count_filter` and `event3_count_filter` uses GaussianMixture model (no hard cutoff). This underestimates. Reports lots of regions to be expressed, although they are not expressed\n",
    "Therefore currently '_median_filter' is used, which uses a hard cutoff. As a cutoff for the GTEx data 50 was chosen|"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "### 2.2) Quantile filter\n",
    "We first use 'quantile_filter', which filters for junctions that are observed in at least 5% of the samples"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 12,
   "metadata": {},
   "outputs": [],
   "source": [
    "ct_psi5 = ct_psi5.quantile_filter()\n",
    "ct_psi3 = ct_psi3.quantile_filter()"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "### 2.3) Infer annotation of junctions from standart annotation\n",
    "This will infer the gene id, gene type as well as if the donor and acceptor site of the junction are annotated in the provided gtf file"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 13,
   "metadata": {},
   "outputs": [
    {
     "data": {
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       "  <thead>\n",
       "    <tr style=\"text-align: right;\">\n",
       "      <th></th>\n",
       "      <th></th>\n",
       "      <th>gene_name</th>\n",
       "      <th>gene_type</th>\n",
       "      <th>novel_junction</th>\n",
       "      <th>weak_site_donor</th>\n",
       "      <th>weak_site_acceptor</th>\n",
       "      <th>transcript_id</th>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>junctions</th>\n",
       "      <th>gene_id</th>\n",
       "      <th></th>\n",
       "      <th></th>\n",
       "      <th></th>\n",
       "      <th></th>\n",
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       "      <td>False</td>\n",
       "      <td>False</td>\n",
       "      <td>False</td>\n",
       "      <td>ENST00000623808.3_2;ENST00000622921.1_2;ENST00...</td>\n",
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       "      <td>ENST00000670780.1_2;ENST00000660797.1_2;ENST00...</td>\n",
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       "      <td>False</td>\n",
       "      <td>False</td>\n",
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       "    <tr>\n",
       "      <th>Y:21903374-21903620:-</th>\n",
       "      <th>ENSG00000012817</th>\n",
       "      <td>KDM5D</td>\n",
       "      <td>protein_coding</td>\n",
       "      <td>False</td>\n",
       "      <td>False</td>\n",
       "      <td>False</td>\n",
       "      <td>ENST00000541639.5_6;ENST00000317961.8_6;ENST00...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>Y:21903743-21905047:-</th>\n",
       "      <th>ENSG00000012817</th>\n",
       "      <td>KDM5D</td>\n",
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       "      <td>False</td>\n",
       "      <td>False</td>\n",
       "      <td>False</td>\n",
       "      <td>ENST00000541639.5_6;ENST00000317961.8_6;ENST00...</td>\n",
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       "    <tr>\n",
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       "      <th>ENSG00000012817</th>\n",
       "      <td>KDM5D</td>\n",
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       "      <td>False</td>\n",
       "      <td>False</td>\n",
       "      <td>False</td>\n",
       "      <td>ENST00000440077.5_4;ENST00000382806.6_3;ENST00...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>Y:21905279-21906270:-</th>\n",
       "      <th>ENSG00000012817</th>\n",
       "      <td>KDM5D</td>\n",
       "      <td>protein_coding</td>\n",
       "      <td>True</td>\n",
       "      <td>False</td>\n",
       "      <td>True</td>\n",
       "      <td>NaN</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>Y:21906439-21906556:-</th>\n",
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       "                                       gene_name       gene_type  \\\n",
       "junctions             gene_id                                      \n",
       "1:763155-764382:+     ENSG00000228794  LINC01128          lncRNA   \n",
       "1:764484-787306:+     ENSG00000228794  LINC01128          lncRNA   \n",
       "1:764484-783033:+     ENSG00000228794  LINC01128          lncRNA   \n",
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       "1:783186-787306:+     ENSG00000228794  LINC01128          lncRNA   \n",
       "...                                          ...             ...   \n",
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       "\n",
       "                                       novel_junction  weak_site_donor  \\\n",
       "junctions             gene_id                                            \n",
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       "...                                               ...              ...   \n",
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       "\n",
       "                                       weak_site_acceptor  \\\n",
       "junctions             gene_id                               \n",
       "1:763155-764382:+     ENSG00000228794               False   \n",
       "1:764484-787306:+     ENSG00000228794               False   \n",
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       "...                                                   ...   \n",
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       "Y:21903743-21905047:- ENSG00000012817               False   \n",
       "Y:21905125-21906270:- ENSG00000012817               False   \n",
       "Y:21905279-21906270:- ENSG00000012817                True   \n",
       "Y:21906439-21906556:- ENSG00000012817               False   \n",
       "\n",
       "                                                                           transcript_id  \n",
       "junctions             gene_id                                                             \n",
       "1:763155-764382:+     ENSG00000228794  ENST00000608189.5_3;ENST00000670780.1_2;ENST00...  \n",
       "1:764484-787306:+     ENSG00000228794  ENST00000623808.3_2;ENST00000622921.1_2;ENST00...  \n",
       "1:764484-783033:+     ENSG00000228794  ENST00000608189.5_3;ENST00000609009.6_3;ENST00...  \n",
       "1:764484-776579:+     ENSG00000228794  ENST00000670780.1_2;ENST00000660797.1_2;ENST00...  \n",
       "1:783186-787306:+     ENSG00000228794  ENST00000670780.1_2;ENST00000661237.1_2;ENST00...  \n",
       "...                                                                                  ...  \n",
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       "Y:21903743-21905047:- ENSG00000012817  ENST00000541639.5_6;ENST00000317961.8_6;ENST00...  \n",
       "Y:21905125-21906270:- ENSG00000012817  ENST00000440077.5_4;ENST00000382806.6_3;ENST00...  \n",
       "Y:21905279-21906270:- ENSG00000012817                                                NaN  \n",
       "Y:21906439-21906556:- ENSG00000012817  ENST00000440077.5_4;ENST00000382806.6_3;ENST00...  \n",
       "\n",
       "[218488 rows x 6 columns]"
      ]
     },
     "execution_count": 13,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "ct_psi5.infer_annotation(gtf_file)"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 14,
   "metadata": {},
   "outputs": [
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       "                                       gene_name       gene_type  \\\n",
       "junctions             gene_id                                      \n",
       "1:762303-783033:+     ENSG00000228794  LINC01128          lncRNA   \n",
       "1:763155-764382:+     ENSG00000228794  LINC01128          lncRNA   \n",
       "1:763229-764382:+     ENSG00000228794  LINC01128          lncRNA   \n",
       "1:763233-764382:+     ENSG00000228794  LINC01128          lncRNA   \n",
       "1:764484-787306:+     ENSG00000228794  LINC01128          lncRNA   \n",
       "...                                          ...             ...   \n",
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       "Y:21906439-21906556:- ENSG00000012817      KDM5D  protein_coding   \n",
       "\n",
       "                                       novel_junction  weak_site_donor  \\\n",
       "junctions             gene_id                                            \n",
       "1:762303-783033:+     ENSG00000228794            True            False   \n",
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       "1:763233-764382:+     ENSG00000228794           False            False   \n",
       "1:764484-787306:+     ENSG00000228794           False            False   \n",
       "...                                               ...              ...   \n",
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       "Y:21906439-21906556:- ENSG00000012817           False            False   \n",
       "\n",
       "                                       weak_site_acceptor  \\\n",
       "junctions             gene_id                               \n",
       "1:762303-783033:+     ENSG00000228794               False   \n",
       "1:763155-764382:+     ENSG00000228794               False   \n",
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       "...                                                   ...   \n",
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       "\n",
       "                                                                           transcript_id  \n",
       "junctions             gene_id                                                             \n",
       "1:762303-783033:+     ENSG00000228794                                                NaN  \n",
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       "...                                                                                  ...  \n",
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       "\n",
       "[218336 rows x 6 columns]"
      ]
     },
     "execution_count": 14,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "ct_psi3.infer_annotation(gtf_file)"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "### 2.4) Generate reference PSI (this will provide the SpliceMap)"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 19,
   "metadata": {},
   "outputs": [],
   "source": [
    "splicemap_5 = ct_psi5.ref_psi5()\n",
    "splicemap_3 = ct_psi3.ref_psi3()"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "#### SpliceMaps contain a dataframe and a name field. \n",
    "#### If no name is provided during SpliceMap generation the filename of the RNA-split read counts will be used. \n",
    "#### The user can define the name of the SpliceMap when reading in the split-read counts: \n",
    "#### SpliceCountTable.read_csv(path_ro_split_read_counts, name='name of SpliceMap')"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 30,
   "metadata": {},
   "outputs": [
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "Brain_Cortex_raw_count_table\n"
     ]
    }
   ],
   "source": [
    "print(splicemap_5.name)"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 26,
   "metadata": {},
   "outputs": [
    {
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       "      <td>0.602888</td>\n",
       "      <td>835</td>\n",
       "      <td>1385</td>\n",
       "      <td>13.5</td>\n",
       "      <td>LINC01128</td>\n",
       "      <td>lncRNA</td>\n",
       "      <td>False</td>\n",
       "      <td>False</td>\n",
       "      <td>False</td>\n",
       "      <td>ENST00000608189.5_3;ENST00000609009.6_3;ENST00...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>1:764484-776579:+</th>\n",
       "      <th>ENSG00000228794</th>\n",
       "      <td>1</td>\n",
       "      <td>764484</td>\n",
       "      <td>776579</td>\n",
       "      <td>+</td>\n",
       "      <td>1:764484:+</td>\n",
       "      <td>1:764484-776579:+;1:764484-783033:+;1:764484-7...</td>\n",
       "      <td>0.090253</td>\n",
       "      <td>125</td>\n",
       "      <td>1385</td>\n",
       "      <td>13.5</td>\n",
       "      <td>LINC01128</td>\n",
       "      <td>lncRNA</td>\n",
       "      <td>False</td>\n",
       "      <td>False</td>\n",
       "      <td>False</td>\n",
       "      <td>ENST00000670780.1_2;ENST00000660797.1_2;ENST00...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>1:783186-787306:+</th>\n",
       "      <th>ENSG00000228794</th>\n",
       "      <td>1</td>\n",
       "      <td>783186</td>\n",
       "      <td>787306</td>\n",
       "      <td>+</td>\n",
       "      <td>1:783186:+</td>\n",
       "      <td>1:783186-784863:+;1:783186-787306:+</td>\n",
       "      <td>0.705502</td>\n",
       "      <td>654</td>\n",
       "      <td>927</td>\n",
       "      <td>8.0</td>\n",
       "      <td>LINC01128</td>\n",
       "      <td>lncRNA</td>\n",
       "      <td>False</td>\n",
       "      <td>False</td>\n",
       "      <td>False</td>\n",
       "      <td>ENST00000670780.1_2;ENST00000661237.1_2;ENST00...</td>\n",
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       "    <tr>\n",
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       "      <td>...</td>\n",
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       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>Y:21903374-21903620:-</th>\n",
       "      <th>ENSG00000012817</th>\n",
       "      <td>Y</td>\n",
       "      <td>21903374</td>\n",
       "      <td>21903620</td>\n",
       "      <td>-</td>\n",
       "      <td>Y:21903620:-</td>\n",
       "      <td>Y:21901548-21903620:-;Y:21903374-21903620:-</td>\n",
       "      <td>0.993396</td>\n",
       "      <td>1053</td>\n",
       "      <td>1060</td>\n",
       "      <td>10.0</td>\n",
       "      <td>KDM5D</td>\n",
       "      <td>protein_coding</td>\n",
       "      <td>False</td>\n",
       "      <td>False</td>\n",
       "      <td>False</td>\n",
       "      <td>ENST00000541639.5_6;ENST00000317961.8_6;ENST00...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>Y:21903743-21905047:-</th>\n",
       "      <th>ENSG00000012817</th>\n",
       "      <td>Y</td>\n",
       "      <td>21903743</td>\n",
       "      <td>21905047</td>\n",
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       "      <td>Y:21905047:-</td>\n",
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       "      <td>1.000000</td>\n",
       "      <td>1151</td>\n",
       "      <td>1151</td>\n",
       "      <td>10.0</td>\n",
       "      <td>KDM5D</td>\n",
       "      <td>protein_coding</td>\n",
       "      <td>False</td>\n",
       "      <td>False</td>\n",
       "      <td>False</td>\n",
       "      <td>ENST00000541639.5_6;ENST00000317961.8_6;ENST00...</td>\n",
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       "    <tr>\n",
       "      <th>Y:21905125-21906270:-</th>\n",
       "      <th>ENSG00000012817</th>\n",
       "      <td>Y</td>\n",
       "      <td>21905125</td>\n",
       "      <td>21906270</td>\n",
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       "      <td>Y:21906270:-</td>\n",
       "      <td>Y:21905125-21906270:-;Y:21905279-21906270:-</td>\n",
       "      <td>0.975733</td>\n",
       "      <td>965</td>\n",
       "      <td>989</td>\n",
       "      <td>9.0</td>\n",
       "      <td>KDM5D</td>\n",
       "      <td>protein_coding</td>\n",
       "      <td>False</td>\n",
       "      <td>False</td>\n",
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       "      <td>ENST00000440077.5_4;ENST00000382806.6_3;ENST00...</td>\n",
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       "    <tr>\n",
       "      <th>Y:21905279-21906270:-</th>\n",
       "      <th>ENSG00000012817</th>\n",
       "      <td>Y</td>\n",
       "      <td>21905279</td>\n",
       "      <td>21906270</td>\n",
       "      <td>-</td>\n",
       "      <td>Y:21906270:-</td>\n",
       "      <td>Y:21905125-21906270:-;Y:21905279-21906270:-</td>\n",
       "      <td>0.024267</td>\n",
       "      <td>24</td>\n",
       "      <td>989</td>\n",
       "      <td>9.0</td>\n",
       "      <td>KDM5D</td>\n",
       "      <td>protein_coding</td>\n",
       "      <td>True</td>\n",
       "      <td>False</td>\n",
       "      <td>True</td>\n",
       "      <td>NaN</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>Y:21906439-21906556:-</th>\n",
       "      <th>ENSG00000012817</th>\n",
       "      <td>Y</td>\n",
       "      <td>21906439</td>\n",
       "      <td>21906556</td>\n",
       "      <td>-</td>\n",
       "      <td>Y:21906556:-</td>\n",
       "      <td>Y:21906439-21906556:-</td>\n",
       "      <td>1.000000</td>\n",
       "      <td>812</td>\n",
       "      <td>812</td>\n",
       "      <td>7.0</td>\n",
       "      <td>KDM5D</td>\n",
       "      <td>protein_coding</td>\n",
       "      <td>False</td>\n",
       "      <td>False</td>\n",
       "      <td>False</td>\n",
       "      <td>ENST00000440077.5_4;ENST00000382806.6_3;ENST00...</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "<p>218488 rows × 16 columns</p>\n",
       "</div>"
      ],
      "text/plain": [
       "                                      Chromosome     Start       End Strand  \\\n",
       "junctions             gene_id                                                 \n",
       "1:763155-764382:+     ENSG00000228794          1    763155    764382      +   \n",
       "1:764484-787306:+     ENSG00000228794          1    764484    787306      +   \n",
       "1:764484-783033:+     ENSG00000228794          1    764484    783033      +   \n",
       "1:764484-776579:+     ENSG00000228794          1    764484    776579      +   \n",
       "1:783186-787306:+     ENSG00000228794          1    783186    787306      +   \n",
       "...                                          ...       ...       ...    ...   \n",
       "Y:21903374-21903620:- ENSG00000012817          Y  21903374  21903620      -   \n",
       "Y:21903743-21905047:- ENSG00000012817          Y  21903743  21905047      -   \n",
       "Y:21905125-21906270:- ENSG00000012817          Y  21905125  21906270      -   \n",
       "Y:21905279-21906270:- ENSG00000012817          Y  21905279  21906270      -   \n",
       "Y:21906439-21906556:- ENSG00000012817          Y  21906439  21906556      -   \n",
       "\n",
       "                                        splice_site  \\\n",
       "junctions             gene_id                         \n",
       "1:763155-764382:+     ENSG00000228794    1:763155:+   \n",
       "1:764484-787306:+     ENSG00000228794    1:764484:+   \n",
       "1:764484-783033:+     ENSG00000228794    1:764484:+   \n",
       "1:764484-776579:+     ENSG00000228794    1:764484:+   \n",
       "1:783186-787306:+     ENSG00000228794    1:783186:+   \n",
       "...                                             ...   \n",
       "Y:21903374-21903620:- ENSG00000012817  Y:21903620:-   \n",
       "Y:21903743-21905047:- ENSG00000012817  Y:21905047:-   \n",
       "Y:21905125-21906270:- ENSG00000012817  Y:21906270:-   \n",
       "Y:21905279-21906270:- ENSG00000012817  Y:21906270:-   \n",
       "Y:21906439-21906556:- ENSG00000012817  Y:21906556:-   \n",
       "\n",
       "                                                                                  events  \\\n",
       "junctions             gene_id                                                              \n",
       "1:763155-764382:+     ENSG00000228794                                  1:763155-764382:+   \n",
       "1:764484-787306:+     ENSG00000228794  1:764484-776579:+;1:764484-783033:+;1:764484-7...   \n",
       "1:764484-783033:+     ENSG00000228794  1:764484-776579:+;1:764484-783033:+;1:764484-7...   \n",
       "1:764484-776579:+     ENSG00000228794  1:764484-776579:+;1:764484-783033:+;1:764484-7...   \n",
       "1:783186-787306:+     ENSG00000228794                1:783186-784863:+;1:783186-787306:+   \n",
       "...                                                                                  ...   \n",
       "Y:21903374-21903620:- ENSG00000012817        Y:21901548-21903620:-;Y:21903374-21903620:-   \n",
       "Y:21903743-21905047:- ENSG00000012817                              Y:21903743-21905047:-   \n",
       "Y:21905125-21906270:- ENSG00000012817        Y:21905125-21906270:-;Y:21905279-21906270:-   \n",
       "Y:21905279-21906270:- ENSG00000012817        Y:21905125-21906270:-;Y:21905279-21906270:-   \n",
       "Y:21906439-21906556:- ENSG00000012817                              Y:21906439-21906556:-   \n",
       "\n",
       "                                        ref_psi     k     n  median_n  \\\n",
       "junctions             gene_id                                           \n",
       "1:763155-764382:+     ENSG00000228794  1.000000  1297  1297      13.0   \n",
       "1:764484-787306:+     ENSG00000228794  0.306859   425  1385      13.5   \n",
       "1:764484-783033:+     ENSG00000228794  0.602888   835  1385      13.5   \n",
       "1:764484-776579:+     ENSG00000228794  0.090253   125  1385      13.5   \n",
       "1:783186-787306:+     ENSG00000228794  0.705502   654   927       8.0   \n",
       "...                                         ...   ...   ...       ...   \n",
       "Y:21903374-21903620:- ENSG00000012817  0.993396  1053  1060      10.0   \n",
       "Y:21903743-21905047:- ENSG00000012817  1.000000  1151  1151      10.0   \n",
       "Y:21905125-21906270:- ENSG00000012817  0.975733   965   989       9.0   \n",
       "Y:21905279-21906270:- ENSG00000012817  0.024267    24   989       9.0   \n",
       "Y:21906439-21906556:- ENSG00000012817  1.000000   812   812       7.0   \n",
       "\n",
       "                                       gene_name       gene_type  \\\n",
       "junctions             gene_id                                      \n",
       "1:763155-764382:+     ENSG00000228794  LINC01128          lncRNA   \n",
       "1:764484-787306:+     ENSG00000228794  LINC01128          lncRNA   \n",
       "1:764484-783033:+     ENSG00000228794  LINC01128          lncRNA   \n",
       "1:764484-776579:+     ENSG00000228794  LINC01128          lncRNA   \n",
       "1:783186-787306:+     ENSG00000228794  LINC01128          lncRNA   \n",
       "...                                          ...             ...   \n",
       "Y:21903374-21903620:- ENSG00000012817      KDM5D  protein_coding   \n",
       "Y:21903743-21905047:- ENSG00000012817      KDM5D  protein_coding   \n",
       "Y:21905125-21906270:- ENSG00000012817      KDM5D  protein_coding   \n",
       "Y:21905279-21906270:- ENSG00000012817      KDM5D  protein_coding   \n",
       "Y:21906439-21906556:- ENSG00000012817      KDM5D  protein_coding   \n",
       "\n",
       "                                       novel_junction  weak_site_donor  \\\n",
       "junctions             gene_id                                            \n",
       "1:763155-764382:+     ENSG00000228794           False            False   \n",
       "1:764484-787306:+     ENSG00000228794           False            False   \n",
       "1:764484-783033:+     ENSG00000228794           False            False   \n",
       "1:764484-776579:+     ENSG00000228794           False            False   \n",
       "1:783186-787306:+     ENSG00000228794           False            False   \n",
       "...                                               ...              ...   \n",
       "Y:21903374-21903620:- ENSG00000012817           False            False   \n",
       "Y:21903743-21905047:- ENSG00000012817           False            False   \n",
       "Y:21905125-21906270:- ENSG00000012817           False            False   \n",
       "Y:21905279-21906270:- ENSG00000012817            True            False   \n",
       "Y:21906439-21906556:- ENSG00000012817           False            False   \n",
       "\n",
       "                                       weak_site_acceptor  \\\n",
       "junctions             gene_id                               \n",
       "1:763155-764382:+     ENSG00000228794               False   \n",
       "1:764484-787306:+     ENSG00000228794               False   \n",
       "1:764484-783033:+     ENSG00000228794               False   \n",
       "1:764484-776579:+     ENSG00000228794               False   \n",
       "1:783186-787306:+     ENSG00000228794               False   \n",
       "...                                                   ...   \n",
       "Y:21903374-21903620:- ENSG00000012817               False   \n",
       "Y:21903743-21905047:- ENSG00000012817               False   \n",
       "Y:21905125-21906270:- ENSG00000012817               False   \n",
       "Y:21905279-21906270:- ENSG00000012817                True   \n",
       "Y:21906439-21906556:- ENSG00000012817               False   \n",
       "\n",
       "                                                                           transcript_id  \n",
       "junctions             gene_id                                                             \n",
       "1:763155-764382:+     ENSG00000228794  ENST00000608189.5_3;ENST00000670780.1_2;ENST00...  \n",
       "1:764484-787306:+     ENSG00000228794  ENST00000623808.3_2;ENST00000622921.1_2;ENST00...  \n",
       "1:764484-783033:+     ENSG00000228794  ENST00000608189.5_3;ENST00000609009.6_3;ENST00...  \n",
       "1:764484-776579:+     ENSG00000228794  ENST00000670780.1_2;ENST00000660797.1_2;ENST00...  \n",
       "1:783186-787306:+     ENSG00000228794  ENST00000670780.1_2;ENST00000661237.1_2;ENST00...  \n",
       "...                                                                                  ...  \n",
       "Y:21903374-21903620:- ENSG00000012817  ENST00000541639.5_6;ENST00000317961.8_6;ENST00...  \n",
       "Y:21903743-21905047:- ENSG00000012817  ENST00000541639.5_6;ENST00000317961.8_6;ENST00...  \n",
       "Y:21905125-21906270:- ENSG00000012817  ENST00000440077.5_4;ENST00000382806.6_3;ENST00...  \n",
       "Y:21905279-21906270:- ENSG00000012817                                                NaN  \n",
       "Y:21906439-21906556:- ENSG00000012817  ENST00000440077.5_4;ENST00000382806.6_3;ENST00...  \n",
       "\n",
       "[218488 rows x 16 columns]"
      ]
     },
     "execution_count": 26,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "splicemap_5.df"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 27,
   "metadata": {},
   "outputs": [
    {
     "data": {
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       "      <th></th>\n",
       "      <th>Chromosome</th>\n",
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       "      <td>1:764382:+</td>\n",
       "      <td>1:763155-764382:+;1:763229-764382:+;1:763233-7...</td>\n",
       "      <td>0.014211</td>\n",
       "      <td>19</td>\n",
       "      <td>1337</td>\n",
       "      <td>13.0</td>\n",
       "      <td>LINC01128</td>\n",
       "      <td>lncRNA</td>\n",
       "      <td>False</td>\n",
       "      <td>False</td>\n",
       "      <td>False</td>\n",
       "      <td>ENST00000657175.1_2</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>1:763233-764382:+</th>\n",
       "      <th>ENSG00000228794</th>\n",
       "      <td>1</td>\n",
       "      <td>763233</td>\n",
       "      <td>764382</td>\n",
       "      <td>+</td>\n",
       "      <td>1:764382:+</td>\n",
       "      <td>1:763155-764382:+;1:763229-764382:+;1:763233-7...</td>\n",
       "      <td>0.015707</td>\n",
       "      <td>21</td>\n",
       "      <td>1337</td>\n",
       "      <td>13.0</td>\n",
       "      <td>LINC01128</td>\n",
       "      <td>lncRNA</td>\n",
       "      <td>False</td>\n",
       "      <td>False</td>\n",
       "      <td>False</td>\n",
       "      <td>ENST00000622921.1_2;ENST00000668541.1_2</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>1:764484-787306:+</th>\n",
       "      <th>ENSG00000228794</th>\n",
       "      <td>1</td>\n",
       "      <td>764484</td>\n",
       "      <td>787306</td>\n",
       "      <td>+</td>\n",
       "      <td>1:787306:+</td>\n",
       "      <td>1:764484-787306:+;1:783186-787306:+;1:784982-7...</td>\n",
       "      <td>0.308194</td>\n",
       "      <td>425</td>\n",
       "      <td>1379</td>\n",
       "      <td>13.0</td>\n",
       "      <td>LINC01128</td>\n",
       "      <td>lncRNA</td>\n",
       "      <td>False</td>\n",
       "      <td>False</td>\n",
       "      <td>False</td>\n",
       "      <td>ENST00000623808.3_2;ENST00000622921.1_2;ENST00...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>...</th>\n",
       "      <th>...</th>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>Y:21901548-21903203:-</th>\n",
       "      <th>ENSG00000012817</th>\n",
       "      <td>Y</td>\n",
       "      <td>21901548</td>\n",
       "      <td>21903203</td>\n",
       "      <td>-</td>\n",
       "      <td>Y:21901548:-</td>\n",
       "      <td>Y:21901548-21903203:-;Y:21901548-21903620:-</td>\n",
       "      <td>0.994243</td>\n",
       "      <td>1209</td>\n",
       "      <td>1216</td>\n",
       "      <td>11.0</td>\n",
       "      <td>KDM5D</td>\n",
       "      <td>protein_coding</td>\n",
       "      <td>False</td>\n",
       "      <td>False</td>\n",
       "      <td>False</td>\n",
       "      <td>ENST00000541639.5_6;ENST00000317961.8_6;ENST00...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>Y:21903374-21903620:-</th>\n",
       "      <th>ENSG00000012817</th>\n",
       "      <td>Y</td>\n",
       "      <td>21903374</td>\n",
       "      <td>21903620</td>\n",
       "      <td>-</td>\n",
       "      <td>Y:21903374:-</td>\n",
       "      <td>Y:21903374-21903620:-</td>\n",
       "      <td>1.000000</td>\n",
       "      <td>1053</td>\n",
       "      <td>1053</td>\n",
       "      <td>10.0</td>\n",
       "      <td>KDM5D</td>\n",
       "      <td>protein_coding</td>\n",
       "      <td>False</td>\n",
       "      <td>False</td>\n",
       "      <td>False</td>\n",
       "      <td>ENST00000541639.5_6;ENST00000317961.8_6;ENST00...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>Y:21903743-21905047:-</th>\n",
       "      <th>ENSG00000012817</th>\n",
       "      <td>Y</td>\n",
       "      <td>21903743</td>\n",
       "      <td>21905047</td>\n",
       "      <td>-</td>\n",
       "      <td>Y:21903743:-</td>\n",
       "      <td>Y:21903743-21905047:-</td>\n",
       "      <td>1.000000</td>\n",
       "      <td>1151</td>\n",
       "      <td>1151</td>\n",
       "      <td>10.0</td>\n",
       "      <td>KDM5D</td>\n",
       "      <td>protein_coding</td>\n",
       "      <td>False</td>\n",
       "      <td>False</td>\n",
       "      <td>False</td>\n",
       "      <td>ENST00000541639.5_6;ENST00000317961.8_6;ENST00...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>Y:21905125-21906270:-</th>\n",
       "      <th>ENSG00000012817</th>\n",
       "      <td>Y</td>\n",
       "      <td>21905125</td>\n",
       "      <td>21906270</td>\n",
       "      <td>-</td>\n",
       "      <td>Y:21905125:-</td>\n",
       "      <td>Y:21905125-21906270:-</td>\n",
       "      <td>1.000000</td>\n",
       "      <td>965</td>\n",
       "      <td>965</td>\n",
       "      <td>9.0</td>\n",
       "      <td>KDM5D</td>\n",
       "      <td>protein_coding</td>\n",
       "      <td>False</td>\n",
       "      <td>False</td>\n",
       "      <td>False</td>\n",
       "      <td>ENST00000440077.5_4;ENST00000382806.6_3;ENST00...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>Y:21906439-21906556:-</th>\n",
       "      <th>ENSG00000012817</th>\n",
       "      <td>Y</td>\n",
       "      <td>21906439</td>\n",
       "      <td>21906556</td>\n",
       "      <td>-</td>\n",
       "      <td>Y:21906439:-</td>\n",
       "      <td>Y:21906439-21906556:-</td>\n",
       "      <td>1.000000</td>\n",
       "      <td>812</td>\n",
       "      <td>812</td>\n",
       "      <td>7.0</td>\n",
       "      <td>KDM5D</td>\n",
       "      <td>protein_coding</td>\n",
       "      <td>False</td>\n",
       "      <td>False</td>\n",
       "      <td>False</td>\n",
       "      <td>ENST00000440077.5_4;ENST00000382806.6_3;ENST00...</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "<p>218336 rows × 16 columns</p>\n",
       "</div>"
      ],
      "text/plain": [
       "                                      Chromosome     Start       End Strand  \\\n",
       "junctions             gene_id                                                 \n",
       "1:762303-783033:+     ENSG00000228794          1    762303    783033      +   \n",
       "1:763155-764382:+     ENSG00000228794          1    763155    764382      +   \n",
       "1:763229-764382:+     ENSG00000228794          1    763229    764382      +   \n",
       "1:763233-764382:+     ENSG00000228794          1    763233    764382      +   \n",
       "1:764484-787306:+     ENSG00000228794          1    764484    787306      +   \n",
       "...                                          ...       ...       ...    ...   \n",
       "Y:21901548-21903203:- ENSG00000012817          Y  21901548  21903203      -   \n",
       "Y:21903374-21903620:- ENSG00000012817          Y  21903374  21903620      -   \n",
       "Y:21903743-21905047:- ENSG00000012817          Y  21903743  21905047      -   \n",
       "Y:21905125-21906270:- ENSG00000012817          Y  21905125  21906270      -   \n",
       "Y:21906439-21906556:- ENSG00000012817          Y  21906439  21906556      -   \n",
       "\n",
       "                                        splice_site  \\\n",
       "junctions             gene_id                         \n",
       "1:762303-783033:+     ENSG00000228794    1:783033:+   \n",
       "1:763155-764382:+     ENSG00000228794    1:764382:+   \n",
       "1:763229-764382:+     ENSG00000228794    1:764382:+   \n",
       "1:763233-764382:+     ENSG00000228794    1:764382:+   \n",
       "1:764484-787306:+     ENSG00000228794    1:787306:+   \n",
       "...                                             ...   \n",
       "Y:21901548-21903203:- ENSG00000012817  Y:21901548:-   \n",
       "Y:21903374-21903620:- ENSG00000012817  Y:21903374:-   \n",
       "Y:21903743-21905047:- ENSG00000012817  Y:21903743:-   \n",
       "Y:21905125-21906270:- ENSG00000012817  Y:21905125:-   \n",
       "Y:21906439-21906556:- ENSG00000012817  Y:21906439:-   \n",
       "\n",
       "                                                                                  events  \\\n",
       "junctions             gene_id                                                              \n",
       "1:762303-783033:+     ENSG00000228794  1:762303-783033:+;1:764484-783033:+;1:776753-7...   \n",
       "1:763155-764382:+     ENSG00000228794  1:763155-764382:+;1:763229-764382:+;1:763233-7...   \n",
       "1:763229-764382:+     ENSG00000228794  1:763155-764382:+;1:763229-764382:+;1:763233-7...   \n",
       "1:763233-764382:+     ENSG00000228794  1:763155-764382:+;1:763229-764382:+;1:763233-7...   \n",
       "1:764484-787306:+     ENSG00000228794  1:764484-787306:+;1:783186-787306:+;1:784982-7...   \n",
       "...                                                                                  ...   \n",
       "Y:21901548-21903203:- ENSG00000012817        Y:21901548-21903203:-;Y:21901548-21903620:-   \n",
       "Y:21903374-21903620:- ENSG00000012817                              Y:21903374-21903620:-   \n",
       "Y:21903743-21905047:- ENSG00000012817                              Y:21903743-21905047:-   \n",
       "Y:21905125-21906270:- ENSG00000012817                              Y:21905125-21906270:-   \n",
       "Y:21906439-21906556:- ENSG00000012817                              Y:21906439-21906556:-   \n",
       "\n",
       "                                        ref_psi     k     n  median_n  \\\n",
       "junctions             gene_id                                           \n",
       "1:762303-783033:+     ENSG00000228794  0.028377    25   881       8.5   \n",
       "1:763155-764382:+     ENSG00000228794  0.970082  1297  1337      13.0   \n",
       "1:763229-764382:+     ENSG00000228794  0.014211    19  1337      13.0   \n",
       "1:763233-764382:+     ENSG00000228794  0.015707    21  1337      13.0   \n",
       "1:764484-787306:+     ENSG00000228794  0.308194   425  1379      13.0   \n",
       "...                                         ...   ...   ...       ...   \n",
       "Y:21901548-21903203:- ENSG00000012817  0.994243  1209  1216      11.0   \n",
       "Y:21903374-21903620:- ENSG00000012817  1.000000  1053  1053      10.0   \n",
       "Y:21903743-21905047:- ENSG00000012817  1.000000  1151  1151      10.0   \n",
       "Y:21905125-21906270:- ENSG00000012817  1.000000   965   965       9.0   \n",
       "Y:21906439-21906556:- ENSG00000012817  1.000000   812   812       7.0   \n",
       "\n",
       "                                       gene_name       gene_type  \\\n",
       "junctions             gene_id                                      \n",
       "1:762303-783033:+     ENSG00000228794  LINC01128          lncRNA   \n",
       "1:763155-764382:+     ENSG00000228794  LINC01128          lncRNA   \n",
       "1:763229-764382:+     ENSG00000228794  LINC01128          lncRNA   \n",
       "1:763233-764382:+     ENSG00000228794  LINC01128          lncRNA   \n",
       "1:764484-787306:+     ENSG00000228794  LINC01128          lncRNA   \n",
       "...                                          ...             ...   \n",
       "Y:21901548-21903203:- ENSG00000012817      KDM5D  protein_coding   \n",
       "Y:21903374-21903620:- ENSG00000012817      KDM5D  protein_coding   \n",
       "Y:21903743-21905047:- ENSG00000012817      KDM5D  protein_coding   \n",
       "Y:21905125-21906270:- ENSG00000012817      KDM5D  protein_coding   \n",
       "Y:21906439-21906556:- ENSG00000012817      KDM5D  protein_coding   \n",
       "\n",
       "                                       novel_junction  weak_site_donor  \\\n",
       "junctions             gene_id                                            \n",
       "1:762303-783033:+     ENSG00000228794            True            False   \n",
       "1:763155-764382:+     ENSG00000228794           False            False   \n",
       "1:763229-764382:+     ENSG00000228794           False            False   \n",
       "1:763233-764382:+     ENSG00000228794           False            False   \n",
       "1:764484-787306:+     ENSG00000228794           False            False   \n",
       "...                                               ...              ...   \n",
       "Y:21901548-21903203:- ENSG00000012817           False            False   \n",
       "Y:21903374-21903620:- ENSG00000012817           False            False   \n",
       "Y:21903743-21905047:- ENSG00000012817           False            False   \n",
       "Y:21905125-21906270:- ENSG00000012817           False            False   \n",
       "Y:21906439-21906556:- ENSG00000012817           False            False   \n",
       "\n",
       "                                       weak_site_acceptor  \\\n",
       "junctions             gene_id                               \n",
       "1:762303-783033:+     ENSG00000228794               False   \n",
       "1:763155-764382:+     ENSG00000228794               False   \n",
       "1:763229-764382:+     ENSG00000228794               False   \n",
       "1:763233-764382:+     ENSG00000228794               False   \n",
       "1:764484-787306:+     ENSG00000228794               False   \n",
       "...                                                   ...   \n",
       "Y:21901548-21903203:- ENSG00000012817               False   \n",
       "Y:21903374-21903620:- ENSG00000012817               False   \n",
       "Y:21903743-21905047:- ENSG00000012817               False   \n",
       "Y:21905125-21906270:- ENSG00000012817               False   \n",
       "Y:21906439-21906556:- ENSG00000012817               False   \n",
       "\n",
       "                                                                           transcript_id  \n",
       "junctions             gene_id                                                             \n",
       "1:762303-783033:+     ENSG00000228794                                                NaN  \n",
       "1:763155-764382:+     ENSG00000228794  ENST00000608189.5_3;ENST00000670780.1_2;ENST00...  \n",
       "1:763229-764382:+     ENSG00000228794                                ENST00000657175.1_2  \n",
       "1:763233-764382:+     ENSG00000228794            ENST00000622921.1_2;ENST00000668541.1_2  \n",
       "1:764484-787306:+     ENSG00000228794  ENST00000623808.3_2;ENST00000622921.1_2;ENST00...  \n",
       "...                                                                                  ...  \n",
       "Y:21901548-21903203:- ENSG00000012817  ENST00000541639.5_6;ENST00000317961.8_6;ENST00...  \n",
       "Y:21903374-21903620:- ENSG00000012817  ENST00000541639.5_6;ENST00000317961.8_6;ENST00...  \n",
       "Y:21903743-21905047:- ENSG00000012817  ENST00000541639.5_6;ENST00000317961.8_6;ENST00...  \n",
       "Y:21905125-21906270:- ENSG00000012817  ENST00000440077.5_4;ENST00000382806.6_3;ENST00...  \n",
       "Y:21906439-21906556:- ENSG00000012817  ENST00000440077.5_4;ENST00000382806.6_3;ENST00...  \n",
       "\n",
       "[218336 rows x 16 columns]"
      ]
     },
     "execution_count": 27,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "splicemap_3.df"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "## 3) Plotting features"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 28,
   "metadata": {},
   "outputs": [],
   "source": [
    "vcf_file = 'vcf_file.vcf.gz'\n",
    "vcf = MultiSampleVCF(vcf_file)\n",
    "\n",
    "junction = '11:47600710-47600820:+'"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "### With 'plot_kn{5,3}', 'plot_psi{5,3}', and 'plot_psi{5,3}_variants' we can visualize outliers"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 29,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/plain": [
       "<AxesSubplot:xlabel='variant', ylabel='ref_psi'>"
      ]
     },
     "execution_count": 29,
     "metadata": {},
     "output_type": "execute_result"
    },
    {
     "data": {
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      "text/plain": [
       "<Figure size 9000x3000 with 3 Axes>"
      ]
     },
     "metadata": {
      "needs_background": "light"
     },
     "output_type": "display_data"
    }
   ],
   "source": [
    "plt.figure(figsize=(30,10), dpi=300)\n",
    "plt.subplot(1, 3, 1)\n",
    "ct_psi5.plot_kn5(junction)\n",
    "\n",
    "plt.subplot(1, 3, 2)\n",
    "ct_psi5.plot_psi5(junction)\n",
    "\n",
    "plt.subplot(1, 3, 3)\n",
    "ct_psi5.plot_psi5_variants(junction, vcf)"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": null,
   "metadata": {},
   "outputs": [],
   "source": []
  }
 ],
 "metadata": {
  "kernelspec": {
   "display_name": "Python [conda env:anaconda-absplice_paper]",
   "language": "python",
   "name": "conda-env-anaconda-absplice_paper-py"
  },
  "language_info": {
   "codemirror_mode": {
    "name": "ipython",
    "version": 3
   },
   "file_extension": ".py",
   "mimetype": "text/x-python",
   "name": "python",
   "nbconvert_exporter": "python",
   "pygments_lexer": "ipython3",
   "version": "3.7.12"
  }
 },
 "nbformat": 4,
 "nbformat_minor": 4
}